DNA replication – 3D

DNA is a molecule made up of two strands twisted around each other in a double
helix shape. Each strand is made up a sequence of four chemical bases represented by the letters A, C, G and T. The two strands are complementary. This means that wherever there’s a T
in one strand there will be in an A in the opposite strand, and wherever there’s a C there will be a G in the other strand. Each strand has a 5′ end and a 3′ end. The two strands run in opposite
directions. This determines how each strand of DNA is replicated. The first step in DNA replication is to separate the two strands. This unzipping is done by an enzyme
called helicase and results in the formation of a
replication fork. The separated strands each provide a
template for creating a new strand of DNA. An enzyme called primase starts the
process. This enzyme makes a small piece of RNA called a primer. This marks the starting point for the
construction of the new strand of DNA. An enzyme called DNA polymerase binds to the primer and will make the new strand of DNA. DNA polymerase can only add DNA bases in one direction, from the 5′ end to the 3′ end. One of the new strands of DNA, the leading strand, is made continuously, the DNA polymerase adding bases one by one in the 5′ to 3′ direction. The other strand, the lagging strand,
cannot be made in this continuous way because it runs in the opposite
direction the DNA polymerase can therefore only
make this strand in a series of small chunks called Okazaki fragments. Each fragment is started with an RNA primer. DNA polymerase then adds a short row of
DNA bases in the 5′ to 3′ direction. The next primer is then added further
down the lagging strand. Another Okazaki fragment is then made and the process is repeated again. Once the new DNA has been made the enzyme exonuclease removes all
the RNA primers from both strands of DNA. Another DNA polymerase enzyme then fills in the gaps that are left behind with DNA. Finally the enzyme DNA ligase seals
up the fragments of DNA in both strands to form a continuous
double strand. DNA replication is described as semi-
conservative because each DNA molecule is made up
of one old, conserved strand of DNA and one new one.

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